Journal: Journal of Oral Microbiology

Article Title: A review of co-culture models to study the oral microenvironment and disease

doi: 10.1080/20002297.2020.1773122

Figure Lengend Snippet: Summary of co-culture methodologies, common protocols employed, and the advantages and disadvantages of each model system.

Article Snippet: In this study, full-thickness oral mucosa models were prepared from decellularised human matrix and compared with collagen-based 3D mucosa models purchased from SkinEthic Laboratories (Nice, France) and MatTek Corporation (Ashland, MA).

Techniques: Cell Culture, Concentration Assay, In Vivo, Transferring

Journal: Journal of Oral Microbiology

Article Title: A review of co-culture models to study the oral microenvironment and disease

doi: 10.1080/20002297.2020.1773122

Figure Lengend Snippet: Common co-culture systems reported in the literature (a) monospecies 2D cell culture with planktonic bacteria applied; (b) multispecies 2D cell culture with planktonic bacteria applied; (c) multispecies 3D cell culture, typically a collagen-based or decellularised matrix containing fibroblasts, with planktonic bacteria applied; and (d) monospecies 2D cell culture with biofilm applied, typically suspended from a well insert.

Article Snippet: In this study, full-thickness oral mucosa models were prepared from decellularised human matrix and compared with collagen-based 3D mucosa models purchased from SkinEthic Laboratories (Nice, France) and MatTek Corporation (Ashland, MA).

Techniques: Co-Culture Assay, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Increased Collagen Crosslinking in Stiff Clubfoot Tissue: Implications for the Improvement of Therapeutic Strategies

doi: 10.3390/ijms222111903

Figure Lengend Snippet: ( A ) Beta-aminopropionitrile (BAPN) reduced collagen crosslinking and insoluble collagen deposition into ECM after 3 weeks in culture. Both the amount of collagen deposited into the ECM and the concentration of the pyridinoline and deoxypyridinoline crosslinks were normalized to the total protein content in the sample. Data presented as mean ± SD. Kruskal–Wallis ANOVA, Dunn’s test; statistical significance: * p = 0.0252, ** p = 0.0028. ( B ) Second harmonic generation (SHG) imaging of collagen type I fibers after 3 weeks in culture. The decrease in the SHG signal depicted in green pseudocolor (upper panel) demonstrates the effect of BAPN on the correct structural assembly of collagen type I. The autofluorescence of the fibroblasts is depicted in red pseudocolor (lower panel). Leica DMi8 confocal microscope, obj. 63× water immersion, Scale bar = 50µm.

Article Snippet: A three-dimensional (3D) in vitro collagen model of ECM (CytoSelect 24-Well Cell Contraction Assay Kit—floating matrix model, CBA-5020, Cell Biolabs, San Diego, CA, USA) was used to describe the potential of BAPN to reduce ECM contractility.

Techniques: Concentration Assay, Imaging, Microscopy

Journal: Pharmaceutics

Article Title: Microfluidic Liver-on-a-Chip for Preclinical Drug Discovery

doi: 10.3390/pharmaceutics15041300

Figure Lengend Snippet: Summary of the application of LoC in drug development.

Article Snippet: Drug toxicity , collagen-based 3D model, integrated biomimetic array chip, cell–extracellular matrix interaction , PHHs , 122 clinical drugs evaluated for liver toxicity , large-scale hepatotoxicity screening , [ ] .

Techniques: In Vitro, High Throughput Screening Assay